石油学报 ›› 2014, Vol. 35 ›› Issue (1): 99-106.DOI: 10.7623/syxb201401011

• 油田开发 • 上一篇    下一篇

聚合物驱后油藏激活内源微生物驱油现场试验

乐建君1, 刘芳2, 张继元1, 柏璐璐1, 王蕊1, 刘晓波2, 侯兆伟1, 伍晓林1   

  1. 1. 大庆油田有限责任公司勘探开发研究院 黑龙江大庆 163712;
    2. 大庆油田有限责任公司第二采油厂 黑龙江大庆 163414
  • 收稿日期:2013-06-11 修回日期:2013-09-19 出版日期:2014-01-25 发布日期:2013-12-09
  • 通讯作者: 乐建君,男,1966年8月生,1986年毕业于大庆石油学院,现为大庆油田有限责任公司勘探开发研究院高级工程师,主要从事微生物提高采收率研究工作。Email:lejj@petrochina.com.cn
  • 作者简介:乐建君,男,1966年8月生,1986年毕业于大庆石油学院,现为大庆油田有限责任公司勘探开发研究院高级工程师,主要从事微生物提高采收率研究工作。Email:lejj@petrochina.com.cn
  • 基金资助:
    国家高技术研究发展计划(863)项目(2009AA063504)、中国石油天然气股份有限公司科学研究与技术开发项目(2008A-1403)资助。

A field test of activation indigenous microorganism for microbial enhanced oil recovery in reservoir after polymer flooding

Le Jianjun1, Liu Fang2, Zhang Jiyuan1, Bai Lulu1, Wang Rui1, Liu Xiaobo2, Hou Zhaowei1, Wu Xiaolin1   

  1. 1. Exploration and Development Research Institute, PetroChina Daqing Oilfield Limited Company, Daqing 163712, China;
    2. No.2 Oil Production Plant, PetroChina Daqing Oilfield Limited Company, Daqing 163414, China
  • Received:2013-06-11 Revised:2013-09-19 Online:2014-01-25 Published:2013-12-09

摘要: 针对聚合物驱后油藏内源微生物的生长特点,对大庆油田萨南开发区南二区东部聚合物驱后典型油藏开展了激活内源微生物驱油现场试验。通过优选由玉米浆干粉、硝酸钠和磷酸氢二铵构成激活剂,与保护剂聚合物交替注入,并运用分子生态学末端限制性片段长度多态性方法分析了内源微生物激活前后群落结构的变化规律,研究了利用内源微生物驱油技术的可行性。现场试验结果表明:聚合物驱后油藏中有明显的产气增压效果;油藏驱动压力的动态变化及代谢产物气体组分CH4和CO2的δ13C同位素含量波动,验证了激活剂的加入促使油藏内源微生物微氧和无氧代谢交替进行;产出液的各项生化监测指标均有明显变化,激活后优势菌群为Pseudomonas、Thauera和Arcobacter,且丰度增高;试验区阶段累计增油3 068.13 t,含水率下降2.2% ,驱油增产效果明显。该试验的成功实施证明了聚合物驱后油藏采用激活内源微生物驱油的工艺方法是可行的,并为同类油藏进一步提高采收率提供了可借鉴的方法和途径。

关键词: 聚合物驱后油藏, 激活剂, 内源微生物, 菌群结构, 现场试验

Abstract: Based on the characteristics of indigenous microbial growth in reservoirs after polymer flooding (RAPFs), a field test was conducted to activate indigenous microorganisms for microbial enhanced oil recovery (MEOR) in representative RAPFs in the east of Nan-2 district in Sanan development zone, Daqing oilfield. The optimized activator, which consisted of corn steep powder, NaNO3, and (NH4)2HPO3, was injected alternately with protectant polymer. The changes in indigenous microbial community structure before and after activation were examined using terminal restriction fragment length polymorphism analysis, in order to determine the feasibility for application of indigenous MEOR technique. Results showed that: there existed a significant gas supercharging effect in the RAPFs; Dynamic changes in the driving pressure of RAPFs and substantial fluctuations in the δ13C isotope contents of microbially produced CH4 and CO2 confirmed that the addition of activator promoted alternative micro-aerobic and anaerobic metabolism of indigenous microorganisms in the reservoir; there were significant changes in the major biochemical parameters of the produced fluid after activation of indigenous microorganisms; the dominant indigenous microbial populations included Pseudomonas, Thauera, and Arcobacter, whose abundance experienced great increases after activation; the incremental oil production was 3 068.13 t while the water content declined by 2.2%, indicating that MEOR application significantly improved the oil recovery. This work proved that it is feasible to activate indigenous microorganisms for MEOR in RAPFs, which provides an alternative approach for further improving oil recovery in similar reservoirs.

Key words: reservoir after polymer flooding, activator, indigenous microorganism, microbial community structure, field test

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