关键词: 微生物提高采收率法, 菌种, 浓度分布, 局部富集, 生物表面活性剂, 驱油机理, 实验观察

Abstract: Bacteria taken the glycolipid surfactant as the main metabolic production and producing a little low-molecular organic acid simultaneously were selected to put on a piece of slide glass.The experiment showed that the bacteria became enrichment on the oil-water interface due to the chemotaxis.During experiments on bacteria in glass box,it could be seen with eyes that fluid muddy on the oil-water interface got thicker and thicker gradually,because of "breeding in situ".The concentration distribution of the products of samples gotten from different distances from the interface was quantitatively measured.The result shows that there are concentration distribution gradients of products.The microbial oil-displacement experiments were programmed in emulation network models.It could be seen that bacteria were prior to entering into the three-phase boundary of pore wall,oil and water.The oil-displacement mechanisms of several techniques were studied,including emulsifying and starting up the remained oil,peeling the oil film off and altering the humidity of the orifice.The integration of these techniques is available for improving oil recovery.

Key words: microbial enhanced oil recovery, bacteria, concentration distribution, local enrichment, bio-surfactant, oil-displacement mechanism, experimental observation