动态驱替内源微生物生长代谢与驱油效率关系

doi:10.7623/syxb202009009

石油学报 ›› 2020, Vol. 41 ›› Issue (9): 1127-1134.DOI: 10.7623/syxb202009009

动态驱替内源微生物生长代谢与驱油效率关系

胡婧¹, 郭辽原¹, 孙刚正¹, 吴晓玲¹, 宋永亭¹, 林军章¹, 汪卫东¹, 曹嫣镔¹, 王增林²

1. 中国石油化工股份有限公司胜利油田分公司石油工程技术研究院山东东营 257000;
2. 中国石油化工股份有限公司胜利油田分公司山东东营 257000

收稿日期:2019-09-12 修回日期:2020-05-22 出版日期:2020-09-25 发布日期:2020-09-29
通讯作者: 孙刚正,男,1980年10月生,2003年获烟台大学学士学位,2010年获中国海洋大学海洋生物学专业理学博士学位,现为中国石油化工股份有限公司胜利油田分公司石油工程技术研究院微生物采油研究中心副研究员,主要从事微生物采油室内激活及现场实施工作。Email:sungangzheng.slyt@sinopec.com
作者简介:胡婧,女,1980年10月生,2003年获陕西师范大学学士学位,2012年获中国石油大学(华东)博士学位,现为中国石油化工集团胜利石油管理局有限公司博士后,主要从事微生物采油微生物菌群方向研究工作。Email:tomatohu@163.com
基金资助:
中国石油化工股份有限公司重大示范工程项目（P18087-4）和中国石油化工集团有限公司胜利油田分公司博士后项目（YKB1812）资助。

Relationship between the growth and metabolism of indigenous microorganisms and oil displacement efficiency during dynamic displacement

Hu Jing¹, Guo Liaoyuan¹, Sun Gangzheng¹, Wu Xiaoling¹, Song Yongting¹, Lin Junzhang¹, Wang Weidong¹, Cao Yanbin¹, Wang Zenglin²

1. Research Institute of Petroleum Engineering, Sinopec Shengli Oilfield Company, Shandong Dongying 257000, China;
2. Sinopec Shengli Oilfield Company, Shandong Dongying 257000, China

Received:2019-09-12 Revised:2020-05-22 Online:2020-09-25 Published:2020-09-29

摘要/Abstract

摘要：

油藏内源微生物的生长代谢活性直接影响内源微生物驱油技术现场实施效果，摇瓶培养及一维物理模拟实验手段无法真实模拟微生物动态驱替过程。利用三维物理模拟实验体系，开展了内源微生物驱油过程中内源微生物群落生长代谢规律研究。研究结果表明：由于多孔介质对激活剂的滞留和吸附作用，三维模拟动态驱替体系中内源微生物群落激活剂消耗C∶N∶P比传统微生物培养的比例低，而且不同生长阶段的组分消耗比例不同；模拟油藏环境的动态驱替体系中，内源微生物群落生长代谢呈现出与传统微生物培养相似的延滞期、对数生长期、稳定期和衰亡期，但生长周期明显延长。内源微生物群落生长代谢规律与生产动态之间存在明显的对应关系，生长延滞期含水无明显变化，对数生长和稳定期含水快速降低，进入衰亡期后驱替效率快速降低，该认识与现场产出液微生物浓度与生产动态变化规律一致。

关键词: 内源微生物驱油, 三维物理模拟, 内源微生物群落, 生长代谢规律, 驱油效率

Abstract:

The growth and metabolic activity of indigenous microorganisms in reservoirs directly affects the on-site implementation effect of indigenous microorganism oil displacement technology. The dynamic microorganism oil displacement process cannot be truly simulated through shaking culture and one-dimensional physical simulation experiments. Using the three-dimensional physical simulation experiment system, a study is carried out on the growth and metabolism of indigenous microbial community during indigenous microbial flooding. This study confirms that due to the retention and adsorption of activators in porous media, the consumption of activators (C∶N∶P)by the indigenous microbial community in the three-dimensionally simulated dynamic oil displacement system is lower than that by traditional microbial cultivation, and the consumption ratio of components is different in various growth stages. The study also confirms that in the dynamic oil displacement system simulating the reservoir environment, the growth and metabolism of the indigenous microbial community show lag phase, logarithmic growth phase, stable phase, and decline phase, which is similar to traditional microbial cultivation; howerver, the growth cycle was significantly extended. There is a clear correspondence between the growth and metabolism laws of indigenous microbial community and the production performance. There is no significant change in water content during the lag growth phase. The water content decreases rapidly during the logarithmic growth and stable phases, and the displacement efficiency decreases rapidly after entering the decline phase. This understanding is consistent with the change laws of microbial concentration of the on-site produced fluid and production performance. As revealed by this study, the macro-growth and metabolism laws of indigenous microbial community during dynamic oil displacement by indigenous microorganisms provide a theoretical basis for analyzing the on-site implementation effect of indigenous microbial flooding and dynamically controlling the technique process during implementation.

Key words: indigenous microbial flooding, three-dimensional physical simulation, indigenous microbial community, growth and metabolism laws, oil displacement efficiency

中图分类号:

TE357.9

胡婧, 郭辽原, 孙刚正, 吴晓玲, 宋永亭, 林军章, 汪卫东, 曹嫣镔, 王增林. 动态驱替内源微生物生长代谢与驱油效率关系[J]. 石油学报, 2020, 41(9): 1127-1134.

Hu Jing, Guo Liaoyuan, Sun Gangzheng, Wu Xiaoling, Song Yongting, Lin Junzhang, Wang Weidong, Cao Yanbin, Wang Zenglin. Relationship between the growth and metabolism of indigenous microorganisms and oil displacement efficiency during dynamic displacement[J]. Acta Petrolei Sinica, 2020, 41(9): 1127-1134.

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动态驱替内源微生物生长代谢与驱油效率关系

Relationship between the growth and metabolism of indigenous microorganisms and oil displacement efficiency during dynamic displacement

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