Abstract: This paper deals with a strain of bacterium Brevibacterium viscogenes nov.sp.74-230,which produces extracellular polysaccharide from crude oil and its fractions.The effects of ages of the inoculum,several kinds of crude oil and its fractions,and contents of crude oil on the synthesis of polysaccharide were investigated.When crude oil was used as the sole carbon source(12%,w/v)in 50 or 240 1 fermentors,8.0g/l of polysaccharide was obtained.The changes of hydrocarbon components after fermentation were analysed.They indicated that the bacterium strain mainly had utilized n-alkane.The fermented gummy solution was diluted and used as a driving fluid in laboratory scale model experiments.When the injection volume corresponds to 20%/ of the pore volume,the secondary oil recovery was enhanced to about 9% of the initial reserves.

摘要： 微生物胞外多糖,例如由葡萄糖生产的甘兰黑腐病黄单胞菌胶(Xanghan gum)已在石油工业中应用.有报告指出,在节杆菌(Arthrobacter)、棒状杆菌(Corynebaeterium)、分枝杆菌(Mycobacterium)、产碱杆菌(Alcaligenes)和短杆菌(Brevibacterium)等属中,有些菌株能利用正-链烷或液腊形成胞外多糖.