胶质降解和生物乳化在稠油降黏中的作用

doi:10.7623/syxb201204019

石油学报 ›› 2012, Vol. 33 ›› Issue (4): 670-675.DOI: 10.7623/syxb201204019

胶质降解和生物乳化在稠油降黏中的作用

齐义彬 1　王大威 2,3　吴萌萌 1　李国强 1　吕　鑫 2,3　马　挺 1

1　南开大学生命科学学院分子微生物学与技术教育部重点实验室　天津　300071；2　中海油研究总院　北京　100027；3　海洋石油高效开发国家重点实验室　北京　100027

收稿日期:2011-07-28 修回日期:2012-02-14 出版日期:2012-07-25 发布日期:2012-08-01
通讯作者: 齐义彬
作者简介:齐义彬，男，1985年12月生，2008年毕业于河北大学，现为南开大学硕士研究生，主要从事微生物提高采收率方面的研究工作。
基金资助:
国家高技术研究发展计划（863）项目（2009AA063502）资助。

Effects of resin degradation and biological emulsification on the viscosity break of heavy oils

QI Yibin 1　WANG Dawei 2,3　WU Mengmeng 1　LI Guoqiang 1　LÜ Xin 2,3　MA Ting 1

Received:2011-07-28 Revised:2012-02-14 Online:2012-07-25 Published:2012-08-01

摘要/Abstract

摘要：

芽孢杆菌QB26是一株高温解烃菌，能以胶质为唯一碳源生长，在以2%胶质为唯一碳源的无机盐培养基中，55℃好氧振荡培养14d，胶质降解量可以达到45.96%；同样培养条件下，该菌发酵液可以较好地乳化等体积的稠油（55℃条件下黏度为1146 mPa·s），稠油降黏率达到66.49%。该菌与一株假单胞菌T-1复配（V_QB26∶ V _T-1=1：1）后作用稠油，可以显著改善稠油的乳化效果（乳化稳定性增强，平均乳化粒径为17.88 μm，减小粒径67.3%），此时的稠油乳化黏度为5.11 mPa·s，仅为初始黏度的0.45%。物理模拟驱油实验结果表明，该体系在均质岩心和非均质岩心中提高模拟油藏的原油采收率分别为14.4%和22.38%，达到了降解胶质和生物乳化的双重降黏效果，大幅度提高了原油的流动性。

关键词: 胶质降解, 降黏, 微生物, 乳化作用, 物模实验

Abstract:

Bacillus sp. QB26 is a strain of thermophilic hydrocarbon-degrading bacteria which can utilize resins as the sole carbon source for growth. In the mineral salt medium(MSM) with 2% resins as the only carbon source, the degradation rate of resins could reach 45.96% after the 14-day aerobic culture at 55℃. Under the same conditions, the QB26 fermentation could perfectly emulsify the same volume of a heavy oil(with the viscosity of 1146 mPa·s at 55℃) by reducing the viscosity by 66.49%. When this oil was degraded by the combination of QB26 and pseudomonas sp.T-1(V _QB26：V _T-1=1：1), we found emulsifying effects improved greatly because the emulsion stability increased and the average partial size was 17.88 μm., being reduced by 67.3%. Meanwhile, the viscosity of the heavy oil was 5.11 mPa·s, only 0.45% of the original viscosity. In addition, experiments of the indoor homogeneous/heterogeneous core physical simulation showed that this system could improve the oil recovery of the simulated reservoir by 14.4% and 22.38%, respectively. These experiments achieved dual effects of viscosity break by means of resin biodegradation and biological emulsion, which can significantly improve the mobility of crude oils.

Key words: resin degradation, viscosity break, microorganism, emulsification, physical simulation test

齐义彬　王大威　吴萌萌　李国强　吕　鑫　马　挺. 胶质降解和生物乳化在稠油降黏中的作用[J]. 石油学报, 2012, 33(4): 670-675.

QI Yibin　WANG Dawei　WU Mengmeng　LI Guoqiang　Lü Xin　MA Ting. Effects of resin degradation and biological emulsification on the viscosity break of heavy oils[J]. Editorial office of ACTA PETROLEI SINICA, 2012, 33(4): 670-675.

参考文献

[1]郭平，李苗，张廷山.CO₂- 微生物开发近海普通稠油可行性分析[J].西南石油大学学报，2007，29(1)：126-129.

Guo Ping,Li Miao,Zhang Tingshan.The feasibility of microorganism compound flooding in production near shore heavy oil fiflds[J].Journal of Southwest Petroleum University,2007, 29(1):126-129.

[2]李凤梅，郭书海，牛之欣，等.稠油降解菌的筛选及其对胶质和沥青质生物降解[J].土壤通报，2006，34(4)：764-767.

Li Fengmei,Guo Shuhai,Niu Zhixin,et al.Isolation of viscous-oil degradative microorganism and biodegradation to resin and asphaltene[J].Chinese Journal of Soil Science,2006,34(4):764-767.

[3]Bogdanov I I.Simulation of the thermal recovery process in a layered reservoir[J].Fluid Dynamics,1994,39(3):398-401.

[4]Wang Wei,Liu Yuzhang,Gu Yongan.Application of a novel polymer system in chemical enhanced oil recovery(EOR)[J].Colloid ＆ Polymer Science,2003,281:1046-1054.

[5]Wang Jun,Ma Ting.Monitoring exogenous and indigenous bacteria by PCR-DGGE technology during the process of microbial enhanced oil recovery[J].Journal of Industrial Microbiology & Biotechnology,2008,35(6):619-628.

[6]Nazina T N.Microbiological investigations of high-temperature horizons of the Kongdian petroleum reservoir in connection with field trial of a biotechnology for enhancement of oil recovery[J].Microbiology,2007,76(3):287-296.

[7]Nelson S J.Stripper well production increased with MEOR treatment[J].Oil Gas,1991,89(1):114-116.

[8]王德民，程杰成，吴军政，等.聚合物驱油技术在大庆油田的应用[J].石油学报，2005，26(1)：74-78.

Wang Demin,Cheng Jiecheng,Wu Junzheng,et al.Application of polymer flooding technology in Daqing oilfield[J].Acta Petrolei Sinica,2005,26(1):74-78.

[9]陈学君.稠油微生物降粘机理及研究进展[J].科技信息，2009(12)：671-672.

Chen Xuejun.The research progress and viscosity reduction mechanism of microbial enhanced heavy oil recovery[J].Science & Technology Information,2009(12):671-672.

[10]黄英，梁凤来，刁虎欣，等.特重原油油藏微生物采油试验[J].油田化学，2002，19(2)：178-181.

Huang Ying,Liang Fenglai,Diao Huxin,et al.Microbial enhanced oil recovery in extra-heavy crude reservoir[J].Oilfield Chemistry,2002,19(2):178-181.

[11]陈玉祥，陈军，潘成松，等.沥青质/胶质影响稠油乳状液稳定性的研究[J].应用化工，2009，38(2)：194-200.

Chen Yuxiang,Chen Jun,Pan Chengsong,et al.Influence of asphaltenes and resins on the stability of heavy crude emulsions[J].Applied Chemical Industry,2009,38(2):194-200.

[12]吴迪，宋辉，王翀，等.原油脱水站油水分离效果的影响因素和改善措施[J].精细与专用化学品，2010，18(12)：42-49.

Wu Di,Song Hui,Wang Chong,et al.The effective factors and improvement of oil-water separation at crude oil dehydration station[J].Fine and Specialty Chemicals,2010,18(12):42-49.

[13]范振中，俞庆森.残酸对原油脱水的影响及处理[J].浙江大学学报:自然科学版，2005，32(5)：546-549.

Fang Zhenzhong,Yu Qingsen.Treatment of residual acid and its influence on the crude oil dehydration[J].Journal of Zhejiang University:Sciences Edition,2005,32(5):546-549.

[14]国家发展和改革委员会.SY/T5119-2008 岩石中可溶有机物及原油族组分分析[S].北京：石油工业出版社，2008.

The National Development and Reform Commission.SY/T5119-2008 The soluble organic matter and oil component analysis in the rock[S].Beijing:Petroleum Industry Press,2008.

[15]崔长征，沈萍，张甲耀，等. Pseudomonas aeruginosa W3产鼠李糖脂的结构表征及理化性质[J].环境科学学报，2010，30(10)：2030-3034.

Cui Changzheng,Shen Ping,Zhang Jiayao,et al.Structural and physicochemical characterization of rhamnolipids produced by pseudomonas aeruginosa W3[J].Acta Scientiae Circumstantiae,2010,30(10):2030-3034.

[16]梁生康,王修林，陆金仁，等.假单胞菌O-2-2产鼠李糖脂的结构表征及理化性质[J].精细化工，2005，22(7)：499-502.

Liang Shengkang,Wang Xiulin,Lu Jinren,et al.Structure characterization and physico-chemical properties of rhamnolipids produced by pseudomonas O-2-2[J].Fine Chemicals,2005,22(7):499-502.

[17]宋智勇，郭辽原，袁书文，等.高温油藏内源微生物的堵调及种群分布[J].石油学报，2010，31(6)：975-979.

Song Zhiyong,Guo Liaoyuan,Yuan Shuwen,et al.Microbial plugging and community distribution of indigenous thermophilic microbes in high-temperature oil reservoirs[J].Acta Petrolei Sinica,2010,31(6):975-979.

[18]郭万奎，石成方，万新德，等.大庆油田聚合物驱后微生物调剖先导性现场试验研究[J].石油学报，2006，27(增刊):86-90.

Guo Wankui,Shi Chengfang,Wan Xinde,et al.Study on pilot test of microbial profile modification after polymer flooding in Daqing oilfield[J].Acta Petrolei Sinica,2006,27(Supplement):86-90.

[19]Fuchsluger C,Preims M.Automated measurement and quantification of heterotrophic bacteria in water samples based on the MPN method[J].Microbiol Biotechnol,2011,38:241-247.

[20]蒋璐璐，张瑜，刘飞，等.润滑油黏度可见/近红外光谱快速无损检测方法[J].石油学报:石油加工，2011，27(1)：112-116.

Jiang Lulu,Zhang Yu,Liu Fei,et al.Fast and nondestructive detection for viscosity of lubricating oil using visible/near infrared spectroscopy[J].Acta Petrolei Sinica：Petroleum Processing Section,2011,27(1):112-116.

[21]黄威东，王清海，朱道乾，等.在线毛细管柱SFC/FTIR联用方法[J].光谱学与光谱分析，1996,16（4）：37-40.

Huang Weidong,Wang Qinghai,Zhu Daoqian,et al.On-line capillary SFC/FTIR method[J].spectroscopy and Spectral Analysis,1996,16(4):37-40.

胶质降解和生物乳化在稠油降黏中的作用

Effects of resin degradation and biological emulsification on the viscosity break of heavy oils

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