Abstract: Using microorganisms in-situ fermentation to enhance oil recovery is a new technologyin the petroleum industry. This paper discusses the screening and evaluation of bacterialspecies both in the laboratory and in a pilot test in Daqing oilfield. The bacteria used are Pseuodomonas aeruginosa,Xanthemonas compestris,Bacillus licheniforms and 5GA,which is most likely to be Bacteroikes.Oil recovery in the laboratorytests were increased by 34.3%, and the residual oil recovery increase was 69.8%.Ahuff-and-puff pilot test on two wells, Dong 6-J22 and Dong 5-J18,which are located in the heavyoil zones in the oil-water transitional zone of eastern Daqing was run in June-July,1990.After the two wells were shut in for 40 and 64 days,respectively the pressure in the tubingand casing of Well Dong 6-J22 had increased by 1.6MPa and 1.9MPa,respectively. Alsodaily liquid production was increased by 15-20t/d,water cut decreased from 94% to 84%,and oil production increased from 3t/d-5-6t/d and lasted about 8 months. After Well Dong5-J18 was shut in for 64 days,liquid production increased,oil production increased from 7.6Vd to 10-11t/d, and gas production increased from 234 m¹/d to 547 m¹/d and lasted about18 months.

Key words: microbial production, bacteria, fermentation degradation, crude oil, EOR, Daqing oilfield

摘要： 本文系统地报导了大庆油田室内筛选和评价菌种的方法,所采用的假单胞杆菌(Pseuodomonas aeruginosa)、野油菜花黄单胞杆菌(Xanthemonas compestris)、地衣芽孢杆菌(Bacillus Licheniformis)和5GA近似于节杆菌(Bacteroides)的配伍菌种,室内实验提高采收率34.3%,提高残余油采收率69.8%.

关键词: 微生物采油, 细菌, 发酵降解, 原油, 提高采收率, 大庆油田